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METHYLENE BLUE CAS#61-73-4

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Chemical Name：METHYLENE BLUE

CAS No.:61-73-4

Molecular Formula: C16H18ClN3S

Molecular weight: 319.85

Sample: Available

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Product Details

Products Description of METHYLENE BLUE CAS#61-73-4

Methylene blue, a classic biological staining agent. It is used in microbiology and histology to observe cell structures and clearly distinguish pathogens from tissue morphology. The staining effect is excellent and it is one of the essential basic reagents in laboratories.

METHYLENE BLUE CAS#61-73-4

Methylene Blue Chemical Properties

Melting point	190 °C (dec.)(lit.)
density	1.0 g/mL at 20 °C
refractive index	n20/D1.347
Fp	45 °C
storage temp.	room temp
solubility	Soluble in water, ethanol, ethylene glycol, methyl cellosolve
form	Liquid
pka	2.6, 11.2(at 25℃)
Colour Index	52015
color	Green
Specific Gravity	0.98
Odor	Odorless
Water Solubility	40 g/L (20 ºC)
λmax	661 nm
Merck	14,6060
BRN	3641570
Biological Applications	Detecting microorganisms; treating diabetic retinopathy,macular degeneration,malignant uveal melanomas,erysipelas,hidradenitis suppurativa,inflammation,skin diseases
NIST Chemistry Reference	Methylene blue(61-73-4)
EPA Substance Registry System	Methylene blue (61-73-4)

Safety Information

Hazard Codes	Xn
Risk Statements	10-41-22-20/21/22-36/37/38
Safety Statements	26-39-36
RIDADR	UN 1993 3/PG 3
WGK Germany	3
RTECS	SP5740000
HS Code	32049010
Hazardous Substances Data	61-73-4(Hazardous Substances Data)
Toxicity	LD50 oral in rat: 1180mg/kg

Product Application of METHYLENE BLUE CAS#61-73-4

A non-toxic dye, the oxidized form is blue and the reduced form is colorless. When staining yeast living cells, due to cell metabolism, the cells have strong reducing ability, which can change methylene blue from the blue oxidized form to Colorless chemical book type of reduction, which has weak metabolic effect or dead cells, has no or extremely weak reducing ability, and is dyed blue or light blue by methylene blue. Not only can this method be used to observe the morphology of yeast cells, but it can also be used to observe the morphology of yeast cells. To identify dead and live cells of yeast.

It can be used for dyeing linen, silk fabrics, paper, and coloring bamboo and wood. It can also be used in the manufacture of inks and lakes, as well as in the dyeing of biological and bacterial tissues. It can be mixed with basic purple 5BN and yellow dextrin in a ratio of 78:13:9 to form basic royal blue.

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